代答一下
論文中他們是用等鞭金藻(第0~30天做水色及餵水蚤)、朝鮮矮隆哲水蚤(Bestiolina coreana)無節幼蟲(第2~15天)與橈足幼蟲＆成蟲(第10~30天)、以及豐年蝦無節幼蟲(第25~55天)餵食
“Cultures of the microalga Isochrysis galbana were maintained in 80-L photobioreactors with continuous strong aeration. The culture water was filtered to 1 μm, sterilized with chlorine, and enriched with a commercial fertilizer (Hyponex 5 medium at 0.015 g/L). A photoperiod of 14 h light: 10 h dark was maintained using LED lighting at approximately 4000 lx. The cultured microalgae were used as feed for copepods and for water conditioning in fish larviculture. The copepod Bestiolina coreana (strain: NTOU-Best.-1) was originally isolated from Tapong Bay, Taiwan, and has been maintained at the Laboratory of Aquatic Live Feed Organisms at National Taiwan Ocean University since 2021. Copepods were cultured in 2000-L fiber- reinforced plastic (FRP) tanks containing 1-μm filtered seawater and fed daily with I. galbana at 104 to 105 cells/mL. The culture water was renewed weekly by 30 % of total volume. Copepod nauplii (size: 80–100 μm) were harvested for larval feeding by sieving through 120-μm mesh (to retain copepodites and adults) and 38-μm mesh (to collect nauplii). Nauplii of brine shrimp Artemia franciscana (Ocean Star International, USA) were obtained from cysts incubation in a 100-L black FRP tank under strong aeration at 28 ± 1◦C and 35 ± 1 ppt. ”
“The larval feeding regime was determined according to larval mouth size measurements obtained during the experimental period, as well as the feeding regime previously reported for the congener Marcia’s anthias P. marcia. The larval feeding regime was as follows: the microalgae I. galbana (5*104 cells/mL) from 0 to 30 dph; copepod B. coreana nauplii (size: ≤ 120 μm, 3 ind./mL) from 2 to 15 dph; copepod B. coreana copepodites and adults (size: 120 to 450 μm, 3 ind./mL) from 10 to 30 dph; nauplii of brine shrimp A. franciscana (size: 450 μm, 3 ind./mL) from 25 to 55 dph. ”
造福台灣魚友~
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